Neuroanatomical tract-tracing techniques that did go viral

Jose L. Lanciego; Floris G. Wouterlood - Neurosciences Department, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain / Department of Anatomy and Neurosciences, Amsterdam University Medical Centers, Amsterdam, The Netherlands

Neuroanatomical tracing methods remain fundamental for elucidating the complexity of brain circuits. During the past decades, the technical arsenal at our disposal has been greatly enriched, with a steady supply of fresh arrivals. This paper provides a landscape view of classical and modern tools for tract-tracing purposes. Focus is placed on methods that have gone viral, i.e., became most widespread used and fully reliable.

How Amira-Avizo Software is used

Slides […] were studied in a confocal laser scanning microscope. Structures were 3D rendered using Amira™ software (Thermo Fisher Scientific).