Rapeseed is one of the most important and widely cultured oilseed crops for food and nonfood purposes worldwide. Neutral lipids are stored in lipid droplets (LDs) as fuel for germination and subsequent seedling growth. Most of the LD detection in seeds was still in 2D levels, and some of the details might have been lost in previous studies. In the present work, the configuration of LDs in seeds was obtained by confocal imaging combined with 3D reconstruction technology in Brassica napus. The size and shape of LDs, LD numbers, cell interval spaces and cell size were observed and compared at 3D levels in the seeds of different materials with high and low oil content. It was also revealed that different cells located in the same tissue exhibited various oil contents according to the construction at the 3D level, which was not previously reported in B. napus. The present work provides a new way to understand the differential in cell populations and enhance the seed oil content at the single cell level within seeds.
Yongtai Yin, Liangxing Guo, Kang Chen, Zhenyi Guo, Hongbo Chao, Baoshan Wang, and Maoteng Licorresponding author - Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, College of Life Science, Shandong Normal University, Jinan, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang
How Amira-Avizo Software is used
With the rapid advance of 3D reconstruction technology, large sets of imaging data processing software such as AMIRA, allow the simultaneous integration of regulatory components within these individual cells in Arabidopsis