Welcome to the Amira-Avizo Software Use Case Gallery

Below you will find a collection of use cases of our 3D data visualization and analysis software. These use cases include scientific publications, articles, papers, posters, presentations or even videos that show how Amira-Avizo Software is used to address various scientific and industrial research topics.

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High-resolution mapping of brain vasculature and its impairment in the hippocampus of Alzheimer’s disease mice

High-resolution mapping of brain vasculature and its impairment in the hippocampus of Alzheimer’s disease mice

Accumulating evidence indicates the critical importance of cerebrovascular dysfunction in the pathogenesis of Alzheimer’s disease (AD). However, systematic comparative studies on the precise brain vasculature of wild-type and AD model mice are still rare. Using an image optimization method for analyzing Micro-Optical Sectioning Tomography (MOST) data, we generated cross-scale whole-brain 3D atlases that cover the entire vascular system from large vessels down to smallest capillaries at ... Read more

Xiaochuan Zhang, Xianzhen Yin, Jingjing Zhang, Anan Li, Hui Gong, Qingming Luo, Haiyan Zhang, Zhaobing Gao, Hualiang Jiang

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An adult brain atlas reveals broad neuroanatomical changes in independently evolved populations of Mexican cavefish

An adult brain atlas reveals broad neuroanatomical changes in independently evolved populations of Mexican cavefish

A shift in environmental conditions impacts the evolution of complex developmental and behavioral traits. The Mexican cavefish, Astyanax mexicanus, is a powerful model for examining the evolution of development, physiology, and behavior because multiple cavefish populations can be compared to an extant and ancestral-like surface population of the same species. Many behaviors have diverged in cave populations of A. mexicanus, and previous studies have shown that cavefish ha... Read more

Cody Loomis, View ORCID ProfileRobert Peuß, James Jaggard, Yongfu Wang, Sean McKinney, Stephen Raftopoulos, Austin Raftopoulos, Daniel Whu, Matthew Green, Suzanne E. McGaugh, Nicolas Rohner, Alex C. Keene, Erik R. Duboue

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A novel approach for the quantification of inhomogeneous 3D current distribution in fuel cell electrodes

A novel approach for the quantification of inhomogeneous 3D current distribution in fuel cell electrodes

The electrode microstructural properties significantly influence the efficiency and durability of many electrochemical devices including solid oxide fuel cells. Despite the possibility of simulating the electrochemical phenomena within real three-dimensional microstructures, the potential of such 3D microstructural information has not yet been fully exploited. We introduce here a completely new methodology for the advanced characterization of inhomogeneous current distribution base... Read more

A.Bertei, V.Yufit, F.Tariq, N.P.Brandon

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Operando Visualization and Multi-scale Tomography Studies of Dendrite Formation and Dissolution in Zinc Batteries

Operando Visualization and Multi-scale Tomography Studies of Dendrite Formation and Dissolution in Zinc Batteries

Alternative battery technologies are required to meet growing energy demands and address the limitations of present technologies. As such, it is necessary to look beyond lithium-ion batteries. Zinc batteries enable high power density while being sourced from ubiquitous and cost-effective materials. This paper presents, for the first time known to the authors, multi-length scale tomography studies of failure mechanisms in zinc batteries with and without commercial microporous separators. In bo... Read more

Vladimir Yufit, Farid Tariq David S. Eastwood Moshiel Biton Billy Wu Peter D. Lee Nigel P. Brandon

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Microstructural evolution and deformation behavior of Al-Cu alloys: A Transmission X-ray Microscopy (TXM) and micropillar compression study

Microstructural evolution and deformation behavior of Al-Cu alloys: A Transmission X-ray Microscopy (TXM) and micropillar compression study

A unique approach to correlating an evolving 3D microstructure in an Al-Cu alloyand its micro-scale mechanical properties has been introduced. Using these nanoscale three-dimensional microstructures derived from Transmission X-rayMicroscopy (TXM), individual contributions from different strengthening mechanisms were quantified. The spatial distribution and morphology of the individual θ′ and θ phases were seen to play an important role in influencing dislocation storage. Uniaxi... Read more

C. Shashank Kaira, Christopher Kantzos, Jason J. Williams, Vincent De Andrade, Francesco De Carlo, Nikhilesh Chawlaa

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Intrinsic dynamics study identifies two amino acids of TIMP-1 critical for its LRP-1-mediated endocytosis in neurons

Intrinsic dynamics study identifies two amino acids of TIMP-1 critical for its LRP-1-mediated endocytosis in neurons

The tissue inhibitor of metalloproteinases-1 (TIMP-1) exerts inhibitory activity against matrix metalloproteinases and cytokine-like effects. We previously showed that TIMP-1 reduces neurite outgrowth in mouse cortical neurons and that this cytokine-like effect depends on TIMP-1 endocytosis mediated by the low-density lipoprotein receptor-related protein-1 (LRP-1). To gain insight into the interaction between TIMP-1 and LRP-1, we considered conformational changes that occur when a ligand bind... Read more

Laurie Verzeaux, Nicolas Belloy, Jessica Thevenard-Devy, Jérôme Devy, Géraldine Ferracci, Laurent Martiny, Stéphane Dedieu, Manuel Dauchez, Hervé Emonard, Nicolas Etique & Emmanuelle Devarenne-Charpentier

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Immunofluorescence Tomography: High-resolution 3-D reconstruction by serial-sectioning of methacrylate embedded tissues and alignment of 2-D immunofluorescence images

Immunofluorescence Tomography: High-resolution 3-D reconstruction by serial-sectioning of methacrylate embedded tissues and alignment of 2-D immunofluorescence images

Immunofluorescence tomography is a high-resolution 3-D reconstruction method based on methacrylate embedding and serial-sectioning, where 2-D images of immuno-stained serial-sections are computationally aligned into image stacks, and the 3-D volume rendered. Butyl-Methyl Methacrylate (BMMA) plastic was adopted as it preserves excellent tissue morphology and can be de-plasticized easily using an organic solvent, which enables immuno-staining of serial-sections without antibody penetration issu... Read more

Parfitt, Geraint J

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Effect of Microstructure of Porous Transport Layer on Performance in Polymer Electrolyte Membrane Water Electrolyser

Effect of Microstructure of Porous Transport Layer on Performance in Polymer Electrolyte Membrane Water Electrolyser

The porous transport layer (PTL) is a vital component of the polymer electrolyte membrane water electrolyser (PEMWE) as it is both a conduit for current distribution and mass transport. This study aims to examine the influence of the microstructure of the PTL on the performance of a PEMWE by the combination of ex-situ and in-situ techniques. Two PTLs with distinctly different mean pore diameter were characterized to determine key properties such as surface morphology, porosity, pore size dist... Read more

Jude O Majasan, Francesco Iacoviello, Paul R Shearing, Dan JL Brett

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Branching morphogenesis of the urinary collecting system in the human embryonic metanephros

Branching morphogenesis of the urinary collecting system in the human embryonic metanephros

An elaborate system of ducts collects urine from all nephrons, and this structure is known as the urinary collecting system (UCS). This study focused on how the UCS is formed during human embryogenesis. Fifty human embryos between the Carnegie stage (CS) 14 and CS23 were selected from the Kyoto Collection at the Congenital Anomaly Research Center of Kyoto University, Japan. Metanephroses, including the UCS, were segmented on serial digital virtual histological sections. Three-dimensional imag... Read more

Hana Ishiyama, Aoi Ishikawa, Haruka Kitazawa, Sena Fujii, Jun Matsubayashi, Shigehito Yamada, Tetsuya Takakuwa

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High-resolution synchrotron-based X-ray microtomography as a tool to unveil the three-dimensional neuronal architecture of the brain

High-resolution synchrotron-based X-ray microtomography as a tool to unveil the three-dimensional neuronal architecture of the brain

The assessment of neuronal number, spatial organization and connectivity is fundamental for a complete understanding of brain function. However, the evaluation of the three-dimensional (3D) brain cytoarchitecture at cellular resolution persists as a great challenge in the field of neuroscience. In this context, X-ray microtomography has shown to be a valuable non-destructive tool for imaging a broad range of samples, from dense materials to soft biological specimens, arisen as a new method fo... Read more

Matheus de Castro Fonseca, Bruno Henrique Silva Araujo, Carlos Sato Baraldi Dias, Nathaly Lopes Archilha, Dionísio Pedro Amorim Neto, Esper Cavalheiro, Harry Westfahl Jr, Antônio José Roque da Silva, Kleber Gomes Franchini

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Laser-matter interactions in additive manufacturing of SS316L and 13-93 bioactive glass revealed by in situ X-ray imaging

Laser-matter interactions in additive manufacturing of SS316L and 13-93 bioactive glass revealed by in situ X-ray imaging

Laser-matter interactions in laser additive manufacturing (LAM) occur on short time scales (10-6 – 10-3 s) and have traditionally proven difficult to characterise. We investigate these interactions during LAM of stainless steel (SS316 L) and 13-93 bioactive glass powders using a custom built LAM process replicator (LAMPR) with in situ and operando synchrotron X-ray radiography. This reveals a range of melt track solidification phenomena as... Read more

Chu Lun Alex Leung, Sebastian Marussi, Michael Towrie, Jesus del Val Garcia, Robert C. Atwood, Andrew J. Bodey, Julian R. Jones, Philip J. Withers, Peter D.Lee

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Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography

Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography

To quantitatively evaluate brain tissue and its corresponding function, knowledge of the 3D cellular distribution is essential. The gold standard to obtain this information is histology, a destructive and labor-intensive technique where the specimen is sliced and examined under a light microscope, providing 3D information at nonisotropic resolution. To overcome the limitations of conventional histology, we use phase-contrast X-ray tomography with optimized optics, reconstruction, and image an... Read more

Mareike Töpperwien, Franziska van der Meer, Christine Stadelmann, and Tim Salditt

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Enhanced mechanical stability of Ni-YSZ scaffold demonstrated by nanoindentation and Electrochemical Impedance Spectroscopy

Enhanced mechanical stability of Ni-YSZ scaffold demonstrated by nanoindentation and Electrochemical Impedance Spectroscopy

The electrochemical performance of Ni-YSZ SOFC anodes can quickly degrade during redox cycling. Mechanical damage at interfaces significantly decreases the number of active triple phase boundaries. This study firstly focuses on the sintering temperature impact on YSZ scaffold mechanical properties. The YSZ scaffold sintered at 1200 °C exhibited 56% Read more

B.Song; E.Ruiz-Trejo; N.P.Brandon

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Synchrotron X-ray tomographic quantification of microstructural evolution in ice cream – a multi-phase soft solid

Synchrotron X-ray tomographic quantification of microstructural evolution in ice cream – a multi-phase soft solid

The microstructure of food affects our sensorial perception, its attractiveness, and the manufactured product’s shelf-life.

Microstructural evolution in soft matter directly influences not only the material’s mechanical and functional properties, but also our perception of that material’s taste. Using synchrotron X-ray tomography and cryo-SEM we investigated the time–temperature evolution of ice cream’s microstructure. This was enabled via three adv... Read more

Enyu Guo, Guang Zeng, Daniil Kazantsev, Peter Rockett, Julian Bent, Mark Kirkland, Gerard Van Dalen, David S. Eastwood, David StJohn and Peter D. Lee

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Quantification of the degradation of Ni-YSZ anodes upon redox cycling

Quantification of the degradation of Ni-YSZ anodes upon redox cycling

Ni-YSZ anodes for Solid Oxide Fuel Cells are vulnerable to microstructural damage during redox cycling leading to a decrease in the electrochemical performance.

 

  • Quantification of redox damage by coupling 3D tomography, EIS and nanoindentation.
  • YSZ fracture, Ni detachment and agglomeration led to irreversible mechanical damage.
  • Ni nanoparticles obtained upon redox cycling improve electrochemical performance.
  • Loss in TPB densi... Read more

Bowen Song, Enrique Ruiz-Trejo, Antonio Bertei, Nigel P.Brandon

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The University of Birmingham uses Avizo software to explore the North Sea as it was 10,000 years ago

The University of Birmingham uses Avizo software to explore the North Sea as it was 10,000 years ago

The IBM Visual and Spatial Technology Centre (VISTA) specialize in large scale data capture, analysis and visualization for the Arts and Humanities at the University of Birmingham, UK. The VISTA Centre supports interdisciplinary academic research and application development for visualization, spatial analysis and imaging using state-ofthe- art technology.

Avizo software is a fundamental tool that provides new opportunities for data... Read more

IBM Visual and Spatial Technology Centre, Institute of Archaeology, Birmingham Archaeology, University of Birmingham, UK

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Exploring hominin and non-hominin primate dental fossil remains with neutron microtomography

Exploring hominin and non-hominin primate dental fossil remains with neutron microtomography

Fossil dental remains are an archive of unique information for paleobiological studies. Computed microtomography based on Xray microfocus sources (X-µCT) and Synchrotron Radiation (SR-µCT) allow subtle quantification at the micron and sub-micron scale of the meso- and microstructural signature imprinted in the mineralized tissues, such as enamel and dentine, through highresolution “virtual histology”. Nonetheless, depending on the degree of alterations undergone during fossiliza... Read more

Clément Zanolli, Laboratory AMIS, UMR 5288, University of Toulouse III - Paul Sabatier, France, and al.

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Enhanced Imaging of Lithium Ion Battery Electrode Materials

Enhanced Imaging of Lithium Ion Battery Electrode Materials

The authors present for the first time a new methodology of contrast enhancement for 3D imaging, including novel advanced quantification, on a commercial Lithium Iron Phosphate (LFP) LiFePO4 cathode. The aim of this work is to improve the quality of the 3D imaging of challenging battery materials by developing methods to increase contrast between otherwise previously poorly differentiated phases. This is necessary to enable capture of the real geometry of electrode microstructures... Read more

Moshiel Biton, Vladimir Yufit, Farid Tariq, Masashi Kishimoto and Nigel Brandon

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Investigation of cycling-induced microstructural degradation in silicon-based electrodes in lithium-ion batteries using X-ray nanotomography

Investigation of cycling-induced microstructural degradation in silicon-based electrodes in lithium-ion batteries using X-ray nanotomography

The microstructural degradation of a composite silicon electrode at different stages in its cycle life was investigated in 3D using X-ray nano-computed tomography. A reconstructed volume of 36 μm × 27 μm × 26 μm from the composite electrode was imaged in its pristine state and after 1, 10 and 100 cycles. Particle fracturing and phase transformation was observed within the electrode with increased cycling. In addition, a distinct, lower X-ray attenuating phase was clearly resolved,... Read more

Oluwadamilola O. Taiwo, Melanie Loveridge, Shane D.Beattie, Donal P.Finegan, Rohit Bhagat, Daniel J.L.Brett, Paul R.Shearing

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Severe hearing loss and outer hair cell death in homozygous Foxo3 knockout mice after moderate noise exposure

Severe hearing loss and outer hair cell death in homozygous Foxo3 knockout mice after moderate noise exposure

Noise induced hearing loss (NIHL) is a disease that affects millions of Americans. Identifying genetic pathways that influence recovery from noise exposure is an important step forward in understanding NIHL. The transcription factor Foxo3 integrates the cellular response to oxidative stress and plays a role in extending lifespan in many organisms, including humans. Here we show that Foxo3 is required for auditory function after noise exposure in a mouse model system, measured by ABR…Read more

Felicia Gilels, Stephen T. Paquette, Holly J. Beaulac, Anwen Bullen & Patricia M. White

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Improving microstructural quantification in FIB/SEM nanotomography

Improving microstructural quantification in FIB/SEM nanotomography

Advanced nanotomographic analysis is still far from routine, and a number of challenges remain in data acquisition and post-processing. In this work, we present a number of techniques to improve the quality of the acquired data, together with easy-to-implement methods to obtain “advanced” microstructural quantifications. The techniques are applied to a solid oxide fuel cell cathode of interest to the electrochemistry community, but the methodologies are easily adaptable to a wide range of... Read more

Joshua A.Taillon, Christopher Pellegrinelli, Yi-Lin Huang, Eric D.Wachsman, Lourdes G. Salamanca-Riba

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